Ninhydrin sweat test: A simple method for detecting antibodies neutralizing botulinum toxin type A
Identifieur interne : 003C38 ( Main/Exploration ); précédent : 003C37; suivant : 003C39Ninhydrin sweat test: A simple method for detecting antibodies neutralizing botulinum toxin type A
Auteurs : Bernhard Voller [Autriche] ; Ekaterina Moraru [Autriche] ; Eduard Auff [Autriche] ; Michael Benesch [Autriche] ; Werner Poewe [Autriche] ; Jörg Wissel [Autriche] ; Jörg Müller [Autriche] ; Tanja Entner [Autriche] ; Hans Bigalke [Allemagne] ; Peter Schnider [Autriche]Source :
- Movement Disorders [ 0885-3185 ] ; 2004-08.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
- Adult, Animals, Antibodies (analysis), Antibodies (blood), Antibodies (pharmacology), Antigen-Antibody Reactions, Bontoxilysin, Botulinum Toxins, Type A (immunology), Botulinum Toxins, Type A (therapeutic use), Dystonic Disorders (drug therapy), Dystonic Disorders (immunology), Female, Hand (physiology), Humans, In Vitro Techniques, Male, Mice, Middle Aged, Nervous system diseases, Neuromuscular Agents (immunology), Neuromuscular Agents (therapeutic use), Neuromuscular Junction (drug effects), Ninhydrin (chemistry), Sweat, Sweat (chemistry), Sweat (immunology), Test method, antibodies, bioassay, botulinum toxin, dystonia, ninhydrin, sweat test.
- MESH :
- chemical , analysis : Antibodies.
- chemical , blood : Antibodies.
- chemical , chemistry : Ninhydrin.
- chemical , immunology : Botulinum Toxins, Type A, Neuromuscular Agents.
- chemical , pharmacology : Antibodies.
- chemical , therapeutic use : Botulinum Toxins, Type A, Neuromuscular Agents.
- chemistry : Sweat.
- drug effects : Neuromuscular Junction.
- drug therapy : Dystonic Disorders.
- immunology : Dystonic Disorders, Sweat.
- physiology : Hand.
- Adult, Animals, Antigen-Antibody Reactions, Female, Humans, In Vitro Techniques, Male, Mice, Middle Aged.
Abstract
Approximately 5% of patients with cervical dystonia receiving repeated botulinum neurotoxin A (BoNT/A) injections develop secondary loss of treatment benefit. Currently available tests to directly detect neutralizing BoNT/A antibodies (BoNT/A‐AB) are either expensive or time consuming. To establish a simple, clinically useful test for antibody detection, we adapted the ninhydrin sweat test (NST). Eighteen dystonic patients with secondary nonresponse and clinically suspected BoNT/A‐AB formation were tested for BoNT/A‐AB in the mouse diaphragm test (MDT). In addition, the size of the anhidrotic area was determined by the NST 21 days after an intradermal dose of 10 U Dysport into the hypothenar region of the left palm. In nine patients, positive BoNT‐AB titers were found in the MDT. There was a significant correlation between the BoNT/A‐AB titers and the anhidrotic area (Spearman's rho = −0.9, P < 0.0001). Both tests provided comparably good results with respect to qualitative antibody detection. In the clinical situation of secondary nonresponse to BoNT/A therapy, the economical NST may be a helpful tool to detect neutralizing BoNT/A‐AB. © 2004 Movement Disorder Society
Url:
DOI: 10.1002/mds.20073
Affiliations:
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Le document en format XML
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M" first="Michael" last="Benesch">Michael Benesch</name><affiliation wicri:level="1"><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Medical Statistics, University of Vienna</wicri:regionArea><wicri:noRegion>University of Vienna</wicri:noRegion></affiliation></author><author><name sortKey="Poewe, Werner" sort="Poewe, Werner" uniqKey="Poewe W" first="Werner" last="Poewe">Werner Poewe</name><affiliation wicri:level="1"><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Neurology, University of Innsbruck</wicri:regionArea><wicri:noRegion>University of Innsbruck</wicri:noRegion><placeName><settlement type="city">Innsbruck</settlement><region nuts="2" type="region">Tyrol (Land)</region></placeName><orgName type="university">Université de médecine d'Innsbruck</orgName></affiliation></author><author><name sortKey="Wissel, Jorg" sort="Wissel, Jorg" uniqKey="Wissel J" first="Jörg" last="Wissel">Jörg Wissel</name><affiliation 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Disord.</title><idno type="ISSN">0885-3185</idno><idno type="eISSN">1531-8257</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2004-08">2004-08</date><biblScope unit="vol">19</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="943">943</biblScope><biblScope unit="page" to="947">947</biblScope></imprint><idno type="ISSN">0885-3185</idno></series><idno type="istex">734A0D9FE2530C498DB424107884CDCF310FF559</idno><idno type="DOI">10.1002/mds.20073</idno><idno type="ArticleID">MDS20073</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0885-3185</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Animals</term><term>Antibodies (analysis)</term><term>Antibodies (blood)</term><term>Antibodies (pharmacology)</term><term>Antigen-Antibody Reactions</term><term>Bontoxilysin</term><term>Botulinum Toxins, Type A (immunology)</term><term>Botulinum Toxins, Type A (therapeutic use)</term><term>Dystonic Disorders (drug therapy)</term><term>Dystonic Disorders (immunology)</term><term>Female</term><term>Hand (physiology)</term><term>Humans</term><term>In Vitro Techniques</term><term>Male</term><term>Mice</term><term>Middle Aged</term><term>Nervous system diseases</term><term>Neuromuscular Agents (immunology)</term><term>Neuromuscular Agents (therapeutic use)</term><term>Neuromuscular Junction (drug effects)</term><term>Ninhydrin (chemistry)</term><term>Sweat</term><term>Sweat (chemistry)</term><term>Sweat (immunology)</term><term>Test method</term><term>antibodies</term><term>bioassay</term><term>botulinum toxin</term><term>dystonia</term><term>ninhydrin</term><term>sweat test</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ninhydrin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Botulinum Toxins, Type A</term><term>Neuromuscular Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antibodies</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Botulinum Toxins, Type A</term><term>Neuromuscular Agents</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Sweat</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Neuromuscular Junction</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Dystonic Disorders</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Dystonic Disorders</term><term>Sweat</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Hand</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Animals</term><term>Antigen-Antibody Reactions</term><term>Female</term><term>Humans</term><term>In Vitro Techniques</term><term>Male</term><term>Mice</term><term>Middle Aged</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Bontoxilysin</term><term>Méthode essai</term><term>Sueur</term><term>Système nerveux pathologie</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Approximately 5% of patients with cervical dystonia receiving repeated botulinum neurotoxin A (BoNT/A) injections develop secondary loss of treatment benefit. Currently available tests to directly detect neutralizing BoNT/A antibodies (BoNT/A‐AB) are either expensive or time consuming. To establish a simple, clinically useful test for antibody detection, we adapted the ninhydrin sweat test (NST). Eighteen dystonic patients with secondary nonresponse and clinically suspected BoNT/A‐AB formation were tested for BoNT/A‐AB in the mouse diaphragm test (MDT). In addition, the size of the anhidrotic area was determined by the NST 21 days after an intradermal dose of 10 U Dysport into the hypothenar region of the left palm. In nine patients, positive BoNT‐AB titers were found in the MDT. There was a significant correlation between the BoNT/A‐AB titers and the anhidrotic area (Spearman's rho = −0.9, P < 0.0001). Both tests provided comparably good results with respect to qualitative antibody detection. In the clinical situation of secondary nonresponse to BoNT/A therapy, the economical NST may be a helpful tool to detect neutralizing BoNT/A‐AB. © 2004 Movement Disorder Society</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Autriche</li></country><region><li>Tyrol (Land)</li></region><settlement><li>Innsbruck</li></settlement><orgName><li>Université de médecine d'Innsbruck</li></orgName></list><tree><country name="Autriche"><noRegion><name sortKey="Voller, Bernhard" sort="Voller, Bernhard" uniqKey="Voller B" first="Bernhard" last="Voller">Bernhard Voller</name></noRegion><name sortKey="Auff, Eduard" sort="Auff, Eduard" uniqKey="Auff E" first="Eduard" last="Auff">Eduard Auff</name><name sortKey="Benesch, Michael" sort="Benesch, Michael" uniqKey="Benesch M" first="Michael" last="Benesch">Michael Benesch</name><name sortKey="Entner, Tanja" sort="Entner, Tanja" uniqKey="Entner T" first="Tanja" last="Entner">Tanja Entner</name><name sortKey="Moraru, Ekaterina" sort="Moraru, Ekaterina" uniqKey="Moraru E" first="Ekaterina" last="Moraru">Ekaterina Moraru</name><name sortKey="Muller, Jorg" sort="Muller, Jorg" uniqKey="Muller J" first="Jörg" last="Müller">Jörg Müller</name><name sortKey="Poewe, Werner" sort="Poewe, Werner" uniqKey="Poewe W" first="Werner" last="Poewe">Werner Poewe</name><name sortKey="Schnider, Peter" sort="Schnider, Peter" uniqKey="Schnider P" first="Peter" last="Schnider">Peter Schnider</name><name sortKey="Wissel, Jorg" sort="Wissel, Jorg" uniqKey="Wissel J" first="Jörg" last="Wissel">Jörg Wissel</name></country><country name="Allemagne"><noRegion><name sortKey="Bigalke, Hans" sort="Bigalke, Hans" uniqKey="Bigalke H" first="Hans" last="Bigalke">Hans Bigalke</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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